期刊
JOURNAL OF PROTEOME RESEARCH
卷 11, 期 6, 页码 3344-3357出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr300139q
关键词
inflammatory bowel disease; OPLS; metabonomics; chemometrics
资金
- Genome Alberta
- Alberta Department of Advanced Education and Technology
- Crohn's and Colitis Foundation of Canada (CCFC)
- Deutsche Forschungsgemeinschaft (DFG)
- Austrian Science Fund [FWF P 22771]
- Austrian National Bank [OeNB 14429]
- F. Lanyar Foundation [351]
- Austrian Science Fund (FWF) [P 22771] Funding Source: researchfish
Serologic biomarkers for inflammatory bowel disease (IBD) have yielded variable differentiating ability. Quantitative analysis of a large number of metabolites is a promising method to detect IBD biomarkers. Human subjects with active Crohn's disease (CD) and active ulcerative colitis (UC) were identified, and serum, plasma, and urine specimens were obtained. We characterized 44 serum, 37 plasma, and 71 urine metabolites by use of H-1 NMR spectroscopy and targeted analysis to differentiate between diseased and non-diseased individuals, as well as between the CD and UC cohorts. We used multiblock principal component analysis and hierarchical OPLS-DA for comparing several blocks derived from the same objects (e.g., subject) to examine differences in metabolites. In serum and plasma of IBD patients, methanol, mannose, formate, 3-methyl-2-oxovalerate, and amino acids such as isoleucine were the metabolites most prominently increased, whereas in urine, maximal increases were observed for mannitol, allantoin, xylose, and carnitine. Both serum and plasma of UC and CD patients showed significant decreases in urea and citrate, whereas in urine, decreases were observed, among others, for betaine and hippurate. Quantitative metabolomic profiling of serum, plasma, and urine discriminates between healthy and IBD subjects. However, our results show that the metabolic differences between the CD and UC cohorts are less pronounced.
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