期刊
JOURNAL OF PROTEOME RESEARCH
卷 11, 期 8, 页码 4249-4260出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr300365f
关键词
ambient pH; Botrytis cinerea; cell wall degrading enzymes; proteomics; secretome
资金
- National Natural Science Foundation of China [31030051, 31171765]
- National High Technology Research (863) Program of China [2012AA101607]
- Chinese Academy of Sciences [KSCX2-EW-G-6]
Botrytis cinerea causes gray mold rot on over 200 plant species worldwide, resulting in great economic loss every year. Cooperation of proteins secreted by B. cinerea plays an important role in its successful infection to host plants. The ambient pH, as one of the most important environmental parameters, can regulate expression of secreted proteins in various fungal pathogens. In the present study, we mainly investigated the effect of ambient pH on secretome of B. cinerea strain B05.10 with a comparative proteomic method based on 2-DE. Distinct differences in secretome of B. cinerea were found between pH 4 and 6 treatments, and 47 differential spots, corresponding to 21 unique proteins, were identified using MALDI-TOF/TOF. At pH 4, more proteins related to proteolysis were induced, whereas most of up-accumulated proteins were cell wall degrading enzymes at pH 6. Analysis of gene expression using quantitative real-time PCR suggests that production of most of these proteins was regulated at the level of transcription. These findings indicate that B. cinerea can adjust protein profile of secretome responding to different ambient pH values and provide evidence to deeply understand the complicated infecting mechanisms of B. cinerea on a wide range of plant hosts.
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