期刊
JOURNAL OF PROTEOME RESEARCH
卷 11, 期 5, 页码 2710-2720出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr2010633
关键词
LPI-FlowCell; mass spectrometry; proteomics; bacterial typing; bacterial identification
资金
- W.R. Wiley Environmental Molecular Science Laboratory
- U.S. Department of Energy's Office of Biological and Environmental Research and located at PNNL
- Battelle Memorial Institute for the U.S. Department of Energy [DE-AC05-76RL0 1830]
- Health & Medical Care Committee of the Regional Executive Board, Region Vastra Gotaland [72241]
- ALF-Medel for Forskning Projects [ALFGBG-11574, ALFGBG-210591]
- FoU-Vastra Gotaland Region Projects [VGFOUREG-30781, 83080, 157801]
- Wellcome Trust
- European Union
- Knut and Alice Wallenberg Foundation [KAW2007.0118]
Because of the alarming expansion in the diversity and occurrence of bacteria displaying virulence and resistance to antimicrobial agents, it is increasingly important to be able to detect these microorganisms and to differentiate and identify closely related species, as well as different strains of a given species. In this study, a mass spectrometry proteomics approach is applied, exploiting lipid-based protein immobilization (LPI), wherein intact bacterial cells are bound, via membrane-gold interactions, within a FlowCell. The bound cells are subjected to enzymatic digestion for the generation of peptides, which are subsequently identified, using LC-MS. Following database matching, strain-specific peptides are used for subspecies-level discrimination. The method is shown to enable a reliable typing and identification of closely related strains of the same bacterial species, herein illustrated for Helicobacter pylori.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据