期刊
JOURNAL OF PROTEOME RESEARCH
卷 12, 期 1, 页码 135-150出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr3008607
关键词
proteins; genes; antibodies; mRNA; mass spectrometry; bioinformatics; protein microarray; human disease
资金
- Swedish Academy of Pharmaceutical Sciences
- Swedish Research Council
- Swedish Foundation for Strategic Research
- Vinnova Ingabritt & Arne Lundbergs forskningsstiftelse
- Crafoord Foundation
- Thermo Fisher Scientific
- Mobilitas Program sponsored by the European Union Social Fund and administered by the Estonian Science Foundation
- Health Institute Carlos III of Spain ISCIII, FIS [PI02114]
- ISCIII [FI08/00721]
- Cancer Prevention and Research Institute of Texas
- University of Texas Medical Branch
- Swedish Cancer Society [11-0624]
- National Cancer Institute [R33 CA 127768-03, R01CA160816, P50-CA92629]
- Sidney Kimmel Center for Prostate and Urologic Cancers
- Prostate Cancer Foundation
- NATIONAL CANCER INSTITUTE [P50CA092629, R01CA160816, R33CA127768] Funding Source: NIH RePORTER
A first research development progress report of the Chromosome 19 Consortium with members from Sweden, Norway, Spain, United States, China and India, a part of the Chromosome-centric Human Proteome Project (C-HPP) global initiative, is presented (http://www.c-hpp.org). From the chromosome 19 peptide-targeted library constituting 6159 peptides, a pilot study was conducted using a subset with 125 isotope-labeled peptides. We applied an annotation strategy with triple quadrupole, ESI-Qtrap, and MALDI mass spectrometry platforms, comparing the quality of data within and in between these instrumental set-ups. LC-MS conditions were outlined by multiplex assay developments, followed by MRM assay developments. SRM was applied to biobank samples, quantifying kallikrein 3 (prostate specific antigen) in plasma from prostate cancer patients. The antibody production has been initiated for more than 1200 genes from the entire chromosome 19, and the progress developments are presented. We developed a dedicated transcript microarray to serve as the mRNA identifier by screening cancer cell lines. NAPPA protein arrays were built to align with the transcript data with the Chromosome 19 NAPPA chip, dedicated to 90 proteins, as the first development delivery. We have introduced an IT-infrastructure utilizing a LIMS system that serves as the key interface for the research teams to share and explore data generated within the project. The cross-site data repository will form the basis for sample processing, including biological samples as well as patient samples from national Biobanks.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据