期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 3, 页码 1416-1419出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr1011476
关键词
Western blot; loading control; Coomassie; MALDI-TOF; PVDF; GAPDH; peptide mass fingerprinting
资金
- Laryngfonden
- Crafoord Foundation
- Magnus Bergvalls Foundation
- Mrs. Berta Kamprad Foundation
- NHS
In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
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