期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 12, 页码 5547-5554出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr200756n
关键词
metabolomics; siderophore; yersiniabactin; Escherichia coli; primary metabolism; ybtU; ybtA; arginine biosynthesis
资金
- National Institutes of Health [HD001459-09, UL1 RR024992, HL101263-01, DK064540-09, DK082315]
- Burroughs Wellcome Fund
Bacterial siderophores may enhance pathogenicity by scavenging iron, but their expression has been proposed to exert a substantial metabolic cost. Here we describe a combined metabolomic-genetic approach to determine how mutations affecting the virulence-associated siderophore yersiniabactin affect the Escherichia coli primary metabolome. Contrary to expectations, we did not find yersiniabactin biosynthesis to correspond to consistent metabolomic shifts. Instead, we found that targeted deletion of ybtU or ybtA, dissimilar genes with similar roles in regulating yersiniabactin expression, were associated with a specific shift in arginine pathway metabolites during growth in minimal media. This interaction was associated with high arginine levels in the model uropathogen Escherichia coli UTI89 compared to its ybtU and ybtA mutants and the K12 strain MG1655, which lacks yersiniabactin-associated genes. Because arginine is not a direct yersiniabactin biosynthetic substrate, these findings show that virulence-associated secondary metabolite systems may shape bacterial primary metabolism independently of substrate consumption.
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