期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 8, 页码 3313-3323出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr2002885
关键词
C-13-Metabolic Flux Analysis; Flux Balance Analysis; Pathway Projector; genome-scale model
资金
- Yamagata Prefecture and Tsuruoka City
- Grants-in-Aid for Scientific Research [22681029, 22860056] Funding Source: KAKEN
One of the ultimate goals of systems biology research is to obtain a comprehensive understanding of the control mechanisms of complex cellular metabolisms. Metabolic Flux Analysis (MFA) is a important method for the quantitative estimation of intracellular metabolic flows through metabolic pathways and the elucidation of cellular physiology. The primary challenge in the use of MFA is that many biological networks are underdetermined systems; it is therefore difficult to narrow down the solution space from the stoichiometric constraints alone. In this tutorial, we present an overview of Flux Balance Analysis (FBA) and C-13-Metabolic Flux Analysis (C-13-MFA), both of which are frequently used to solve such underdetermined systems, and we demonstrate FBA and C-13-MFA using the genome-scale model and the central carbon metabolism model, respectively. Furthermore, because such comprehensive study of intracellular fluxes is inherently complex, we subsequently introduce various pathway mapping and visualization tools to facilitate understanding of these data in the context of the pathways. Specific visualization of MFA results using the BioCyc Omics Viewer and Pathway Projector are shown as illustrative examples.
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