期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 2, 页码 756-762出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr100906v
关键词
Coxiella burnetti; antibiotic resistance; fluoroquinolones; levofloxacin; MS-driven proteomics; COFRADIC
资金
- University of Crete
- Greek Ministry of Education
- General Secretariat for Research and Technology [PENED-03ED863]
- Fund for Scientific Research-Flanders (Belgium) [G.0042.07]
- Ghent University [BOF07/GOA/012]
- Interuniversity Attraction Poles [IUAP06]
The etiological agent of Q fever, Coxiella burnetii, is an obligate intracellular bacterium that multiplies within a phagosome-like parasitophorous vacuole. Fluoroquinolones have been used as an alternative therapy for Q fever. Resistance to fluoroquinolones can arise via several mechanisms utilized by pathogens to avoid killing. Until today, genome-based studies have shown that the main mechanism of C. burnetii to resist inhibition by fluoroquinolones is based on mutations in quinolone-resistance-determining region (QRDR). In this study, in a broader search at the protein level for C. burnetii mechanisms that confer resistance to fluoroquinolones, the proteomes of in vitro developed fluoroquinolone resistant bacteria and susceptible bacteria were compared using the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique. Quantitative comparison of the 381 proteins identified in both strains indicated the different expression of 15 bacterial proteins. These proteins are involved in different cellular processes indicating that the antibiotic resistance mechanism of the bacterium is a multifaceted process.
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