期刊
JOURNAL OF PROTEOME RESEARCH
卷 10, 期 11, 页码 4970-4982出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr200446r
关键词
proteomics; GLUT4; insulin action; NHE6; SILAC; plasma membrane; 3T3-L1 adipocyte
资金
- Australian Government Systemic Infrastructure Initiative
- UNSW
- NHMRC of Australia
- Diabetes Australia Research Trust
The adipocyte is a key regulator of mammalian metabolism. To advance our understanding of this important cell, we have used quantitative proteomics to define the protein composition of the adipocyte plasma membrane (PM) in the presence and absence of insulin. Using this approach, we have identified a high confidence list of 486 PM proteins, 52 of which potentially represent novel cell surface proteins, including a member of the adiponectin receptor family and an unusually high number of hydrolases with no known function. Several novel insulin-responsive proteins including the sodium/hydrogen exchanger, NHE6 and the collagens III and VI were also identified, and we provide evidence of PM-ER association suggestive of a unique functional association between these two organelles in the adipocyte. Together these studies provide a wealth of potential therapeutic targets for the manipulation of adipocyte function and a valuable resource for metabolic research and PM biology.
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