期刊
JOURNAL OF PROTEOME RESEARCH
卷 9, 期 2, 页码 677-688出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr9000866
关键词
Lactococcus lactis; cell envelope; proteome; protein abundance; comparative proteomics; LC-MS/MS; digestive tract; spectral counting; peptide counting; membrane protein
资金
- Commission of the European Communities Marie Curie Project LABHFALTH [MEST-CT-2004-514428]
- Ile de France regional council
- Marie Curie fellowship
We characterized the insoluble proteome of Lactococcus lactis using 1D electrophoresis-LC-MS/MS and identified 313 proteins with at least two different peptides. The identified proteins include 89 proteins having a predicted signal peptide and 25 predicted to be membrane-located. In addition, 67 proteins had alkaline isoelectric point values. Using spectra and peptide counts, we compared protein abundances in two different conditions: growth in rich medium, and after transit in the mouse digestive tract. We identified the large mechanosensitive channel and a putative cation transporter as membrane markers of bacterial adaptation to the digestive tract.
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