期刊
JOURNAL OF PROTEOME RESEARCH
卷 9, 期 2, 页码 1138-1143出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr900816a
关键词
Proteomics; Database search; Precursor estimation; Mass accuracy
资金
- National Institutes of Health [P41 RR011823, S10 RR021026, R01 DK069386, T32 HG00035]
In shotgun proteomics, the analysis of tandem mass spectrometry data from peptides can benefit greatly from high mass accuracy measurements. In this study, we have evaluated two database search strategies which use high mass accuracy measurements of the peptide precursor ion. Our results indicate that peptide identifications are improved when spectra are searched with a wide mass tolerance window and precursor mass is used as a filter to discard incorrect matches. Database searches with a peptide data set constrained to peptides within a narrow mass window resulted in fewer peptide identifications but a significantly faster database search time.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据