期刊
JOURNAL OF PROTEOME RESEARCH
卷 9, 期 2, 页码 990-996出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr900885k
关键词
Neuropeptide; peptidomics; sea urchin; de novo sequencing; genome-wide database search; indexed genome
资金
- SBO [IWT-50164]
- US National Institutes of Health [P30DA018310]
Peptidomics is the identification and study of the in vivo biologically active peptide profile. A combination of high performance liquid chromatography, mass spectrometry, and bioinformatics tools such as database search engines are commonly used to perform the analysis. We report a methodology based on a database system holding the completed translated genome, whereby de novo sequencing and genome-wide database searching are combined. The methodology was applied to the sea urchin neuropeptidome resulting in a 30% increase in identification rate.
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