期刊
JOURNAL OF PROTEOME RESEARCH
卷 9, 期 2, 页码 1144-1149出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr900608z
关键词
proteomics; serum; iTRAQ; 2D LC; peptide libraries
资金
- Canadian Institutes of Health Research
- National Science and Engineering Council of Canada
The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commercial hexapeptide ligand library for quantitative and comparative serum proteomic analysis. We also compared this technology with IgY-based affinity depletion.
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