期刊
JOURNAL OF PROTEOME RESEARCH
卷 9, 期 11, 页码 6077-6081出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr1005923
关键词
Laserspray ionization; multiply charged protein ions; IMS-MS; ESI; MALDI; atmospheric pressure; transmission geometry; imaging mass spectrometry; drift time resolution; isomers
资金
- Wayne State University
- NSF [0955975]
- Waters Company
- DuPont Company
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0955975] Funding Source: National Science Foundation
The ability of laserspray ionization (LSI) to produce multiply charged ions by laser ablation from the solid state, directly from a surface, and at atmospheric pressure allows protein analysis on an ion mobility spectrometry (IMS)-mass spectrometry (MS) instrument (SYNAPT G2) having a mass-to-charge limit of 8000. The matrix, 2,5-dihydroxyacetophenone, lowers the thermal requirements for desolvation of matrix/analyte clusters to produce the highly charged LSI ions under gentle conditions to retain structural integrity of the proteins. Examples include cytochrome C and lysozyme. The solvent-free IMS gas-phase separation is used to baseline separate in the drift time dimension the isomeric solubility restricted beta-amyloid (1-42) from the reversed (42-1). The LSI process is shown to be sufficiently soft to preserve structural integrity and permit separation according to the different shapes. These results suggest that LSI-IMS-MS potentially combines speed of analysis and imaging capability common to matrix-assisted laser desorption/ionization and multiple charging with the potential for structural analysis common to electrospray ionization.
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