期刊
JOURNAL OF PROTEOME RESEARCH
卷 8, 期 3, 页码 1610-1616出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr800986c
关键词
Proteomics; MudPIT; nano-LC; 2D peptide separation; vented column
资金
- Ontario Cancer Institute
- CR Younger Foundation
- Canadian Institutes of Health Research
- Canadian Cancer Society
- National Cancer Institute of Canada
Given the complexity of the mammalian proteome, high-resolution separation technologies are required to achieve comprehensive proteome coverage and to enhance the detection of low-abundance proteins. Among several technologies, Multidimensional Protein Identification Technology (MudPIT) enables the on-line separation of highly complex peptide mixtures directly coupled with mass spectrometry-based identification. Here, we present a variation of the traditional MudPIT protocol, combining highly sensitive chromatography using a nanoflow liquid chromatography system (nano-LC) with a two-dimensional precolumn in a vented column setup. When compared to the traditional MudPIT approach, this nanoflow variation demonstrated better first-phase separation leading to more proteins being characterized while using rather simple instrumentation and a protocol that requires less time and very little technical expertise to perform.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据