期刊
JOURNAL OF PROTEOME RESEARCH
卷 7, 期 1, 页码 328-338出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr070300g
关键词
embryonal carcinoma; mitochondrial biogenesis; quantitative proteomics; cell cycle; neurogenesis
资金
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES012039] Funding Source: NIH RePORTER
- NIEHS NIH HHS [R01 ES012039-03, R01 ES012039, R01 ES012039-02, R01 ES012039-05, R01 ES012039-01, R01-ES012039, R01 ES012039-04] Funding Source: Medline
A quantitative proteomic analysis of changes in protein expression accompanying the differentiation of P19 mouse embryonal carcinoma cells into neuron-like cells using isobaric tag technology coupled with LC-MS/MS revealed protein changes reflecting withdrawal from the cell cycle accompanied by a dynamic reorganization of the cytoskeleton and an up-regulation of mitochondrial biogenesis. Further study of quantitative changes in abundance of individual proteins in a purified mitochondrial fraction showed that most mitochondrial proteins increased significantly in abundance. A set of chaperone proteins did not participate in this increase, suggesting that neuron-like cells are relatively deficient in mitochondrial chaperones. We developed a procedure to account for differences in recovery of mitochondrial proteins during purification of organelles from distinct cell or tissue sources. Proteomic data supported by RT-PCR analysis suggests that enhanced mitochondrial biogenesis during neuronal differentiation may reflect a large increase in expression of PGC-1 alpha combined with down-regulation of its negative regulator, p160 Mybbp1a.
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