期刊
JOURNAL OF PROTEOME RESEARCH
卷 7, 期 11, 页码 4831-4840出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr800403z
关键词
quantitative proteomics; iTRAQ; pulsed-Q dissociation; collision-activated dissociation; ion trap; gastric cancer
资金
- National Cancer Centre Singapore Research Foundation
- Ministry of Education [AcRF: T206B3211]
- Biomedical Research Council [BMRC: 07/1/22/19/531]
Coupling of multiplex isobaric tags for relative and absolute quantitation (iTRAQ) to a sensitive linear ion trap (LTQ) mass spectrometer (MS) is a challenging, but highly promising approach for quantitative high-throughput proteomic profiling. Integration of the advantages of pulsed-Q dissociation (PQD) and collision-activated dissociation (CAD) fragmentation methods into a PQD-CAD hybrid mode, together with PQD optimization and data manipulation with a bioinformatics algorithm, resulted in a robust, sensitive and accurate iTRAQ quantitative proteomic workflow. The workflow was superior to the default PQD setting when profiling the proteome of a gastric cancer cell line, SNU5. Taken together, we established an optimized PQD-CAD hybrid workflow in LTQ-MS for iTRAQ quantitative proteomic profiling that may have wide applications in biological and biomedical research.
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