期刊
JOURNAL OF PROTEOME RESEARCH
卷 7, 期 4, 页码 1683-1692出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr7006973
关键词
fractionation; saponin; CMC; pores; mel-5; 2D gels; 2D-LC-MS/MS; TOF-SIMS; soluble biomarkers
Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据