期刊
PHYSIOLOGIA PLANTARUM
卷 156, 期 2, 页码 215-226出版社
WILEY
DOI: 10.1111/ppl.12387
关键词
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资金
- National Basic Research Program of China [2013CB127403]
- National Natural Science Foundation of China [31172020, 31272236]
- Innovative Research Team Development Plan of the Ministry of Education of China [IRT1256]
- Graduate research and innovation projects in Jiangsu Province [KYLX_0562]
- China Postdoctoral Science Foundation [2015M571768]
- Jiangsu Postdoctoral Science Foundation [1402148C]
Our previous studies demonstrated that chloroplastic CO2 concentration (Cc) is not sufficient under high-nitrogen (N) supply in rice plants. In this research, we studied how aquaporins-(AQPs) mediated Cc under different N-supply levels. A hydroponic experiment was conducted in a greenhouse with three different N levels (low N, 0.71 mM; intermediate N, 2.86 mM; and high N, 7.14 mM) in a rice cultivar (Oryza sativa cv. Shanyou 63) and with an ospip1; 1 mutant (Oryza sativa cv. Nipponbare). The photosynthetic nitrogen-use efficiency (PNUE) decreased with increasing leaf-N content. Under high-N supply, the estimated Cc was significantly lower than the theoretical Cc and the specific Rubisco activity (carboxylation efficiency/Rubisco content, CE/Rubisco) decreased, because of a decrease of relative CO2 diffusion conductance (total CO2 diffusion conductance/leaf-N content, gt/N) in mesophyll cells. Real Time Quantitative PCR (Q-RT-PCR) showed that most OsPIP1s and OsPIP2s expression were downregulated under the high-N supply. Furthermore, Cc and g(m) decreased in the ospip1; 1 mutant line compared with that of the wild-type plant. It was concluded that under high-N supply, the decreased PNUE was associated with non-sufficient Cc, mediated by AQP in mesophyll conductance.
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