期刊
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
卷 47, 期 17, 页码 4302-4313出版社
WILEY
DOI: 10.1002/pola.23482
关键词
dendrimers; dendritic carbohydrate; functionalization of polymers; poly(N-(2-hydroxypropyl) methacrylamide); pyridyl disulfide; reversible addition fragmentation chain transfer (RAFT); short interfering RNAs; telechelics
资金
- Australian Research Council (ARC)
Well-defined pyridyl disulfide (PDS) end-functionalized polymer-dendritic carbohydrate scaffolds are reported as novel precursors for the attachment of biomolecules. This synthetic approach combines reversible addition fragmentation chain transfer (RAFT) polymerization and click reactions. Poly(N-(2-hydroxypropyl) methacrylamide) (PHPMA) with 2-mercaptothiozalidine end-groups was prepared by RAFT polymerization yielding molecular weights of M-n = 4300 and 9900, both with a polydispersity of less than 1.2. These polymers were then attached to dendritic mannose scaffolds preconstructed via consecutive click reactions. Finally, the omega-dithiobenzoate RAFT end-group of PHPMA was modified to yield PDS functionality, by aminolysis in the presence of 2,2'-dithiodipyridine. This PDS end-functionalized PHPMA-dendritic carbohydrate scaffold is a versatile precursor for bioconjugations, as the synthetic procedure can easily accommodate a range of sugar functionalities. In addition, the PDS groups can be used to react with any thiol present in a biomolecule (e.g., cysteine residue in proteins, or -SH terminal nucleotides). To demonstrate the utility of these scaffolds we describe their bioconjugation to short interfering RNA. (C) 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 4302-4313, 2009
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