期刊
JOURNAL OF PLANT RESEARCH
卷 124, 期 3, 页码 395-403出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10265-010-0377-7
关键词
Vacuole; Tobacco BY-2; Vacuolar sucrose transporter
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [19039008]
- Institute for Bioinformatics Research and Development (BIRD) from Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [23012009, 19039008, 22114501, 22114505] Funding Source: KAKEN
Sucrose plays an important role in several cellular processes since it is a general source of metabolic energy, serves as a precursor for starch and cellulose synthesis, and is a metabolic starting point for carboxylate- and amino acid synthesis. While plant vacuole is the main cellular storage pool, where sucrose accumulates to high concentrations, only a small number of vacuolar sugar transporters have been identified and characterized to date. We initially identified a vacuolar sucrose transporter (NtSUT4) from tobacco BY-2 cells and established transgenic tobacco BY-2 cell lines that overexpress NtSUT4-GFP (BY-SUTG cells). Using a model system for synchronous cell elongation in miniprotoplasts (evacuolated cells) prepared from tobacco BY-2 cells, we found that NtSUT4-GFP overexpression inhibited cell growth towards the cell major axis. Moreover, under the same conditions, we found that the cell walls were well stained by calcofluor in BY-SUTG cells than in wild type BY-2 cells. These results suggest that NtSUT4 is involved in cell shape via sucrose homeostasis in plant cells.
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