Chlorophyll a fluorescence induction (Kautsky curve) in a Venus flytrap (Dionaea muscipula) leaf after mechanical trigger hair irritation

This paper describes experiments on transient changes in chlorophyll a fluorescence in traps of the carnivorous plant Venus flytrap (Dionaea muscipula) that occur in association with mechanical stimulation of trigger hairs and propagation of action potentials (APs). The experiments show the following reproducible effects of APs on the fluorescence induction (Kautsky-, or OJIPSMT curve) in a 100 s low intensity light pulse (i) no change in the OJ phase attributed to release of photochemical quenching, (ii) a small enhancement, if at all of increase in the thermal JIP phase, (iii) a two- to threefold deceleration of the fluorescence decline (quenching) during the PSMT phase in the 2-100 s time range, and (iv) a transient 15-50% increase in variable fluorescence within similar to 20 s under steady state light condition with, after similar to 80 s, a 10% undershoot that reverses in several tens of seconds to the original steady state. The results are discussed in terms of a hypothesis that the fluorescence decline during the SMT phase of the Kautsky induction curve, attributed to NPQ is caused by the Delta mu(H+)-driven increase in proton conductance of the CFo channel of the ATPase during its activation. A signal-transducing role of Ca2+ is suggested. (C) 2012 Elsevier GmbH. All rights reserved.