期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 169, 期 15, 页码 1501-1510出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2012.06.008
关键词
Abscisic acid; Hydrogen peroxide; Mitogen-activated protein kinase kinase; Osmotic stress; Transgenic tobacco
资金
- Nation Natural Science Foundation of China [30871457, 31071337]
- State Key Basic Research and Development Plan of China [2009CB118500]
Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling modules and function as a convergent point for crosstalk during abiotic stress signaling. In this article, we isolated a novel group B MAPKK gene, ZmMKK3, from Zea mays. ZmMKK3 protein might be localized in both the cytoplasm and the nucleus. RNA blot analysis indicated that the ZmMKK3 transcription was up-regulated by abscisic acid (ABA), hydrogen peroxide (H2O2) and PEG, and that H2O2 mediated PEG-induced expression of ZmMKK3. Constitutive expression of ZmMKK3 in Nicotiana tabacum reduced H2O2 accumulation under osmotic stress by affecting antioxidant defense systems and alleviated reactive oxygen species-mediated injury under oxidative stress. Transgenic tobacco exhibited attenuated ABA sensitivity by means of an increased germination rate and main root growth. Taken together, these results indicate that ZmMKK3 is a positive regulator of osmotic tolerance and ABA signaling in plants. (C) 2012 Elsevier GmbH. All rights reserved.
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