期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 169, 期 6, 页码 621-627出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2011.12.008
关键词
Basic helix-loop-helix transcription factor; Defence response; Jasmonic acid; Oryza sativa L. cv. Nipponbare; Chitinase
资金
- Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- Grants-in-Aid for Scientific Research [23580145] Funding Source: KAKEN
The plant hormone jasmonic acid (JA) is known to be involved in multiple defence responses against pathogens, which include the production of pathogenesis-related (PR) proteins. In order to investigate the induction mechanism of the rice defence responses by JA, we performed transcriptome analyses and focused on a chitinase gene, OsChia4a, which was identified to be one of the highest JA-inductive genes. The recombinant protein of His-tagged OsChia4a exhibited an inhibitory effect against the spore germination and hyphal growth of Magnaporthe oryzae. The promoter analysis of OsChia4a revealed that the region from -515 bp to -265 bp upstream of the ATG translation initiation site was required for the responsiveness to JA. A subsequent mutation analysis indicated that an E-box (CANNTG) in this region act as a JA-responsive cis element. These results imply that a basic helix-loop-helix transcription factor is likely to be involved in the regulation of the OsChia4a expression in a JA-dependent manner. (C) 2011 Elsevier GmbH. All rights reserved.
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