期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 168, 期 9, 页码 960-966出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2010.11.023
关键词
Cell elongation; Drought resistance; Expansin gene; Transgenic tobacco; Wheat
资金
- National Natural Science Foundation of China [30671259]
Expansins are proteins that are the key regulators of wall extension during plant growth. To investigate the role of TaEXPB23, a wheat expansin gene, we analyzed TaEXPB23 mRNA expression levels in response to water stress in wheat and examined the drought resistance of transgenic tobaccos over-expressing TaEXPB23. We found that the expression of TaEXPB23 corresponded to wheat coleoptile growth and the response to water stress. The results also indicated that the transgenic tobacco lines lost water more slowly than the wild-type (WT) plants under drought stress; their cells could sustain a more integrated structure under water stress than that of WT. Other physiological and biochemical parameters under water stress, such as electrolyte leakage, malondialdehyde (MDA) level, photosynthetic rate, F-v/F-m and Phi PSII, also suggested that the transgenic tobaccos were more drought resistant than WT plants. (C) 2011 Elsevier GmbH. All rights reserved.
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