期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 168, 期 8, 页码 848-852出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2010.11.006
关键词
Beta caryophyllene; Biofuel; Cyanobacterium; Sesquiterpene
资金
- University of Northern Colorado
- Colorado office of Economic Development
The plant secondary metabolite, beta-caryophyllene, is a ubiquitous component of many plant resins that has traditionally been used in the cosmetics industry to provide a woody, spicy aroma to cosmetics and perfumes. Clinical studies have shown it to be potentially effective as an antibiotic, anesthetic, and anti-inflammatory agent. Additionally, there is significant interest in engineering phototrophic microorganisms with sesquiterpene synthase genes for the production of biofuels. Currently, the isolation of beta-caryophyllene relies on purification methods from oleoresins extracted from large amounts of plant material. An engineered cyanobacterium platform that produces beta-caryophyllene may provide a more sustainable and controllable means of production. To this end, the beta-caryophyllene synthase gene (QHS1) from Artemisia annua was stably inserted, via double homologous recombination, into the genome of the cyanobacterium Synechocystis sp. strain PCC6803. Gene insertion into Synechocystis was confirmed through PCR assays and sequencing reactions. Transcription and expression of QHS1 were confirmed using RT-PCR, and synthesis of beta-caryophyllene was confirmed in the transgenic strain using GC-FID and GC-MS analysis. (C) 2010 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据