期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 166, 期 9, 页码 968-977出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2008.11.008
关键词
Carotenogenesis; Dunaliella salina; 4-Hydroxy-3-methylbut-2-enyl diphosphate reductase; Isoprenoid biosynthesis; Methylerythritol phosphate pathway
资金
- Fundaqao para a Ciencia a Tecnologia, Portugal [SFRH/BD/13937/2003, SFRH/BD/4839/2001]
- OVERCAROTEN [POCTI/MAR/15237/99]
- INTERREG159-SAL-Atlantic Salt Ponds
- Portuguese national budget
In green algae, the final step of the plastidial methylerythritol phosphate (MEP) pathway is catalyzed by 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR; EC: 1.17.1.2), an enzyme proposed to play a key role in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 1959-bp Dunaliella salina HDR (DsHDR) cDNA encoding a deduced polypeptide of 474 amino acid residues. Phylogenetic analysis implied a cyanobacterial origin for plant and algal HDR genes. Steady-state DsHDR transcript levels were higher in D. solina cells submitted to nutritional depletion, high salt and/or high light, suggesting that DsHDR may respond to the same environmental cues as genes involved in carotenoid biosynthesis. (C) 2008 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据