期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 165, 期 7, 页码 734-744出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.jplph.2007.03.010
关键词
endochitinase gene; European chestnut; hydrogen peroxide; in vitro shoots; wound-signaling pathways
Salicylic acid (SA), ethylene (ET), and wounding are all known to influence plant defense response. Experiments attempting to determine SA's relation to ET biosynthesis and defense gene expression have shown conflicting results. To confront this, we developed an in vitro model system to investigate how SA affects ET biosynthesis, hydrogen peroxide (H(2)O(2)) production and endochitinase gene expression in the European chestnut. ET measurements of in vitro shoots indicated a critical time point for SA exogenous application, enabling us to study its effects independent of ET. In addition, ET measurements demonstrated that its own increased biosynthesis was a response to wounding but not to SA treatment. Application of the ET biosynthesis inhibitor, aminoethoxyvinylglycine (AVG), on wounded and SA-treated shoots blocked wounding-induced ET production. Interestingly, SA inhibited ET production, but to a lesser extent than AVG. Additionally, SA also induced the accumulation of endochitinase transcript level. Likewise, a sensitive tissue-print assay showed that SA further increased the level of H(2)O(2). Yet, SA-induced endochitinase gene expression and SA-enhanced H(2)O(2) production levels were independent of ET The cumulative results indicate that SA acts as an inducer of endochitinase PR gene expression and of H(2)O(2) oxidative burst. This suggests that SA is a component of the signal. transduction pathway leading to defense against pathogens in chestnut. Further, the model. system developed for this experiment should facilitate the deciphering of defense signaling pathways and their cross-talk. Moreover, it should also benefit the study of trees of long generation time that are known to be recalcitrant to in vitro studies. (c) 2007 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据