期刊
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
卷 23, 期 1, 页码 119-122出版社
SPRINGER INDIA
DOI: 10.1007/s13562-013-0193-y
关键词
Allele-specific marker; Dehydration stress; Foxtail millet; Marker-aided breeding; SiDREB2. Single marker regression; Single nucleotide polymorphism
资金
- Department of Biotechnology, Govt. of India, New Delhi [BT/PR9851/AGR/02/521/2007]
- National Institute of Plant Genome Research, New Delhi, India
- Department of Science & Technology, Govt. of India [IFA-11LSPA-01]
Dehydration-responsive element binding (DREB) genes assist in improving stress tolerance of plants by activating the expression of several stress-responsive genes. Therefore, development of functional markers for useful alleles utilizing DREB genes is crucial for crop improvement strategies. Earlier we reported a synonymous single nucleotide polymorphism (SNP) associated with dehydration tolerance at 558(th) bp (an A/G transition) in the SiDREB2 gene of foxtail millet (Setaria italica L.) and developed an allele-specific marker (ASM) for SiDREB2. In the present study, we validated this ASM using a set of 122 foxtail accessions, of which 45 were investigated in an earlier study. The QTL associated with SiDREB2 contributed to similar to 20 % of the total phenotypic variation (PV) for relative water content (RWC) and this signified the importance of this QTL for dehydration tolerance in foxtail millet.
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