期刊
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
卷 19, 期 1, 页码 99-102出版社
SOC PLANT BIOCHEM BIOTECH
DOI: 10.1007/BF03323444
关键词
Plasmid DNA; Extraction protocol; Xanthomonas axonopodis pv. malvacearum; Escherichia coli
Efficient protocols developed to isolate low copy plasmid DNA from Xanthomonas axonopodis pv malvacearum (Xam) and high copy recombinant plasmid DNA from Escherichia coli are described. The protocol for extraction of low copy plasmid DNA from strains of Xam yielded high concentrations of plasmid DNA and used easily available and inexpensive chemicals in simple steps. The protocol for plasmid extraction from E. coli was rapid, cost-effective and yet yielded high concentrations of plasmid DNA. The procedures are simple and can be used to process several samples at one time. The plasmid DNA extracted by two methods was sufficiently pure, free from protein and other cellular contaminants and amenable to various molecular manipulations.
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