期刊
JOURNAL OF PINEAL RESEARCH
卷 57, 期 4, 页码 393-407出版社
WILEY
DOI: 10.1111/jpi.12179
关键词
melatonin; melatonin receptor 2; motility; mouse skin wound; umbilical cord blood-derived mesenchymal stem cells
资金
- Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea [A120216]
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009090]
Melatonin, a circadian rhythm-promoting molecule, has a variety of biological functions, but the functional role of melatonin in the motility of mesenchymal stem cells (MSCs) has yet to be studied. In a mouse skin excisional wound model, we found that transplantation of umbilical cord blood (UCB)-MSCs pretreated with melatonin enhanced wound closure, granulation, and re-epithelialization at mouse skin wound sites, where relatively more UCB-MSCs which were engrafted onto the wound site were detected. Thus, we identified the signaling pathway of melatonin, which affects the motility of UCB-MSCs. Melatonin (1m) significantly increased the motility of UCB-MSCs, which had been inhibited by the knockdown of melatonin receptor 2 (MT2). We found that Gq coupled with MT2 and that the binding of Gq to MT2 uniquely stimulated an atypical PKC isoform, PKC. Melatonin induced the phosphorylation of FAK and paxillin, which were concurrently downregulated by blocking of the PKC activity. Melatonin increased the levels of active Cdc42 and Arp2/3, and it has the ability to stimulate cytoskeletal reorganization-related proteins such as profilin-1, cofilin-1, and F-actin in UCB-MSCs. Finally, a lack of MT2 expression in UCB-MSCs during a mouse skin transplantation experiment resulted in impaired wound healing and less engraftment of stem cells at the wound site. These results demonstrate that melatonin signaling via MT2 triggers FAK/paxillin phosphorylation to stimulate reorganization of the actin cytoskeleton, which is responsible for Cdc42/Arp2/3 activation to promote UCB-MSCs motility.
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