期刊
JOURNAL OF PINEAL RESEARCH
卷 52, 期 3, 页码 305-311出版社
WILEY
DOI: 10.1111/j.1600-079X.2011.00944.x
关键词
antioxidant; apoptosis; embryonic development; melatonin; mouse 2-cell embryos; reactive oxygen species; vitrification
资金
- Program for New Century Excellent Talents in University [NETC-10-0782]
- National Natural Science Foundation of China [31172177]
- [2009 ZX08010-020B]
- [2011BAD19B02-4]
- [2008ZX08008-005]
Two-cell embryos of mouse were vitrified by the open-pulled straw (OPS) method. The vitrified embryos were warmed and introduced into M16 medium for culture that contains melatonin at different concentrations (10-3, 10-5, 10-7, 10-9, 10-11 m). This process caused reactive oxygen species (ROS) formation and jeopardized the development of the embryos. Melatonin, at different concentrations, significantly suppresses ROS production and promotes embryonic development in vitrified embryos compared with untreated ones. The mechanistic studies indicated that the beneficial effects of melatonin on vitrified 2-cell embryos of mouse were melatonin receptor (MT1 and MT2) independent. The direct free radical scavenging activity, the enhancement of endogenous glutathione levels, and the anti-apoptotic capacity of melatonin may account for its protective effects on vitrified embryonic development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据