期刊
JOURNAL OF PINEAL RESEARCH
卷 54, 期 2, 页码 170-178出版社
WILEY
DOI: 10.1111/jpi.12002
关键词
dental papilla cell; differentiation; melatonin; mitochondria; proliferation
资金
- National Natural Science Foundation of China [30472248]
- Science Technology Program of Guangdong [2008B030301102]
Melatonin mediates a variety of biological processes ranging from the control of circadian rhythms to immune regulation. Melatonin also influences bone formation and osteointegration of dental implants. However, the effects of melatonin on dentine formation have not been examined. This study investigated the effects of melatonin on the proliferation and differentiation of rat dental papilla cells (rDPCs) in vitro and dentine formation in vivo. We found that melatonin (0, 1012, 1010,108m) induced a dose-dependent reduction in rDPCs proliferation, increased alkaline phosphatase (ALP) activity, the expression of dentine sialoprotein (DSP), and mineralized matrix formation in vitro. In vivo melatonin (50mg/kg, BW, i.p.) inhibited dentine formation. Melatonin (108m) suppressed the activity of complex I and IV in the basal medium (OS) and enhanced the activity of complex I and complex IV in osteogenic medium (OS+). These results demonstrate that melatonin suppresses the proliferation and promotes differentiation of rDPCs, the mechanisms of which may be related to activity of mitochondrial complex I and complex IV.
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