期刊
JOURNAL OF PINEAL RESEARCH
卷 46, 期 2, 页码 188-198出版社
WILEY
DOI: 10.1111/j.1600-079X.2008.00647.x
关键词
melatonin; oxidative phosphorylation; oxygen consumption; oxygen radicals; respiratory chain; uncoupling
资金
- Instituto de Salud Carlos III [PI03/0817, RD06/0013/0008]
- Consejeria de Innovacion, Ciencia y Empresa, Junta de Andalucia [P07-CTS-03135, CTS-101]
- Instituto de Salud Carlos III (Spain)
- University of Granada, Spain
The role of melatonin in improving mitochondrial respiratory chain activity and increasing ATP production in different experimental conditions has been widely reported. To date, however, the mechanism(s) involved are largely unknown. Using high-resolution respirometry, fluorometry and spectrophotometry we studied the effects of melatonin on normal mitochondrial functions. Mitochondria were recovered from mouse liver cells and incubated in vitro with melatonin at concentrations ranging from 1 nm to 1 mm. Melatonin decreased oxygen consumption concomitantly with its concentration, inhibited any increase in oxygen flux in the presence of an excess of ADP, reduced the membrane potential, and consequently inhibited the production of superoxide anion and hydrogen peroxide. At the same time it maintained the efficiency of oxidative phosphorylation and ATP synthesis while increasing the activity of the respiratory complexes (mainly complexes I, III, and IV). The effects of melatonin appeared to be due to its presence within the mitochondria, since kinetic experiments clearly showed its incorporation into these organelles. Our results support the hypothesis that melatonin, together with hormones such as triiodothyronine, participates in the physiological regulation of mitochondrial homeostasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据