期刊
JOURNAL OF PHYTOPATHOLOGY
卷 163, 期 5, 页码 331-341出版社
WILEY
DOI: 10.1111/jph.12256
关键词
Eggplant mottled dwarf virus; Iran; nucleorhabdoviruses; Rhabdoviridae
资金
- Agriculture and Natural Resources Research Center of Shahrekord
- National Science Foundation [MCB-1157906]
- Division of Plant Protection of Shahrekord
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1157906] Funding Source: National Science Foundation
The full-length nucleotide sequence of the Iranian isolate of Eggplant mottled dwarf virus (EMDV), a phytorhabdovirus, was determined using the random polymerase chain reaction method (rPCR) followed by PCR with specific primers to fill in the gaps. The negative-sense RNA genome of the Iranian isolate of EMDV contains 13154 nucleotides and seven open-reading frames (ORFs) in the order 3-leader-N-X-P-Y-M-G-L-trailer-5. These ORFs encode the nucleocapsid, X protein (of unknown function), phosphoprotein, Y protein (putative movement protein), matrix protein, glycoprotein and RNA-dependent RNA polymerase, respectively. EMDV has a 199 nt 3 leader RNA and a 151 nt 5 trailer, and the ORFs are separated by conserved intergenic sequences. Phylogenetic analyses indicate that EMDV is most closely related to Potato yellow dwarf virus, which has a distinctly different geographical distribution.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据