Complete Genome Sequence and Genome Analysis of Eggplant mottled dwarf virus-Iranian Isolate

The full-length nucleotide sequence of the Iranian isolate of Eggplant mottled dwarf virus (EMDV), a phytorhabdovirus, was determined using the random polymerase chain reaction method (rPCR) followed by PCR with specific primers to fill in the gaps. The negative-sense RNA genome of the Iranian isolate of EMDV contains 13154 nucleotides and seven open-reading frames (ORFs) in the order 3-leader-N-X-P-Y-M-G-L-trailer-5. These ORFs encode the nucleocapsid, X protein (of unknown function), phosphoprotein, Y protein (putative movement protein), matrix protein, glycoprotein and RNA-dependent RNA polymerase, respectively. EMDV has a 199 nt 3 leader RNA and a 151 nt 5 trailer, and the ORFs are separated by conserved intergenic sequences. Phylogenetic analyses indicate that EMDV is most closely related to Potato yellow dwarf virus, which has a distinctly different geographical distribution.