期刊
JOURNAL OF PHYTOPATHOLOGY
卷 160, 期 7-8, 页码 418-423出版社
WILEY
DOI: 10.1111/j.1439-0434.2012.01921.x
关键词
Fig; viruses; cDNA; PCR; sequencing; phylogenetic analysis; Turkey
资金
- Mustafa Kemal University-BAP [01M0112]
- TUBITAK, Turkey [1110827]
Several viruses infecting fig trees in Turkey have been identified recently. The samples were collected from the commonest fig cultivars showing typical mosaic symptoms and from symptomless plants from different fig growing regions of Turkey. They were tested for Fig leaf mottle-associated virus 1-2 (FLMaV1-2), Fig mosaic virus (FMV), Fig latent virus-1 (FLV-1), Fig mild mottle-associated virus (FMMaV), Arkansas fig closterovirus 1-2 (AFCV1-2), Fig badnavirus-1 (FBV-1) and Fig cryptic virus (FCV) by PCR and sequence analyses. One hundred fig trees were tested, and 83% of tested samples were found to be infected by at least one virus. Complex infections were detected in most of the samples, and the most common viruses were FBV-1 and FMV with 82 and 79% infection ratios, respectively. The sequence analyses confirmed virus identity except for AFCV-1 for which no sequence data are available in GenBank. Based on phylogenetic analysis, the sequences clustered into seven groups: FLV-1, FMMaV, FBV-1, FCV, FMV, AFCV-1, FLMaV-1, as expected, and no correlation was found between Turkish isolates depending on cultivars and provinces for these viruses.
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