期刊
JOURNAL OF PHYTOPATHOLOGY
卷 159, 期 11-12, 页码 799-801出版社
WILEY
DOI: 10.1111/j.1439-0434.2011.01843.x
关键词
phytoplasma; Rose Balsam phyllody; PCR; RFLP; China
资金
- Education Ministry of China [B07049]
- National Natural Science Foundation of China [30871625]
In July 2010, symptoms suggestive of phytoplasma infection were observed on Rose Balsam (Impatiens balsamina) around Yangling, China. Nested polymerase chain reaction with universal 16S rDNA phytoplasma primers P1/P6 and R16F2n/R16R2 yielded amplicons of expected size (1.2 kb) from all symptomatic, but not asymptomatic, leaf samples. Sequencing results and NCBI BLASTn analysis of the 1246 bp products (R16F2n/R16R2) showed that the phytoplasma belonged to group 16SrI. Restriction fragment length polymorphism and phylogenetic analysis showed the phytoplasma had a close relationship to subgroup 16SrI-D. This is the first report of a phytoplasma infecting Rose Balsam.
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