Cloning and expression analysis of a putative ABC transporter gene BgABC1 from the biotrophic pathogenic fungus Blumeria graminis f. sp tritici

A putative ATP-binding cassette (ABC) transporter gene (BgABC1) was isolated from the biotrophic pathogenic fungus Blumeria graminis f. sp. tritici (Bgt). Analysis of the deduced amino acid sequence of BgABC1 showed that the BgABC1 protein had a conserved nucleotide-binding fold in the N-terminus, and six transmembrane domains (TMDs) in the C-terminus. Analysis of the BgABC1 expression using real-time polymerase chain reaction showed that expression of the gene was increased significantly when the fungus was growing in wheat seedlings treated with 14 alpha-demethylase-inhibiting fungicide, triadimefon. The results indicated that the BgABC1 was involved in the protection of the fungus against fungicide toxicity.