期刊
JOURNAL OF PHYTOPATHOLOGY
卷 156, 期 7-8, 页码 489-492出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1439-0434.2007.01378.x
关键词
membrane filter; Fusarium oxysporum; germination assay; root exudate
The effect of different membrane filters on the bioactivity of tomato root exudates was tested in an in vitro assay addressing the germination of microconidia of the soil-borne fungus Fusarium oxysporum f. sp. lycopersici. Membrane filtration of unsterile root exudates with filters of different membrane materials and filter brands resulted in an increased microconidia germination. This effect varied depending on the used membrane filter but was lacking when sterile root exudates were used. The alteration of the bioactivity of unsterile root exudates therefore seems to be due to the presence of microbial contaminants. The varying effects of different filter brands may be due to their differential potential of retaining inhibitory compounds. When working with root exudates, such effects of membrane filtration have to be taken into account.
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