期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 592, 期 14, 页码 3013-3030出版社
WILEY
DOI: 10.1113/jphysiol.2014.271718
关键词
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资金
- Harrison Department of Surgical Research, Department of Surgery, University of Pennsylvania
- National Institutes of Health [DK-077699]
- National Disease Research Interchange (NDRI)
- NIH [5U42 RR006042]
The mechanisms of mechanosensitivity underlying the response of the human bladder to stretch are poorly understood. Animal data suggest that stretch-activated two-pore-domain (K-2P) K+ channels play a critical role in bladder relaxation during the filling phase. The objective of this study was to characterize the expression and function of stretch-activated K-2P channels in the human bladder and to clarify their physiological role in bladder mechanosensitivity. Gene and protein analysis of the K-2P channels TREK-1, TREK-2 and TRAAK in the human bladder revealed that TREK-1 is the predominantly expressed member of the mechano-gated subfamily of K-2P channels. Immunohistochemical labelling of bladder wall identified higher levels of expression of TREK-1 in detrusor smooth muscle cells in comparison to bladder mucosa. Functional characterization and biophysical properties of the predominantly expressed member of the K-2P family, the TREK-1 channel, were evaluated by in vitro organ bath studies and the patch-clamp technique. Electrophysiological recordings from single smooth muscle cells confirmed direct activation of TREK-1 channels by mechanical stretch and negative pressure applied to the cell membrane. Inhibition of TREK-1 channels in the human detrusor significantly delayed relaxation of the stretched bladder smooth muscle strips and triggered small-amplitude spontaneous contractions. Application of negative pressure to cell-attached patches (-20 mmHg) caused a 19-fold increase in the open probability (NPo) of human TREK-1 channels. L-Methionine (1 mM), a specific TREK-1 inhibitor, dramatically decreased the NPo of TREK-1 channels from 0.045 +/- 0.003 to 0.008 +/- 0.001 (n = 8, P <= 0.01). Subsequent addition of arachidonic acid (10 mu M), a channel opener, increased the open probability of methionine- inhibited unitary currents up to 0.43 +/- 0.05 at 0 mV (n = 9, P <= 0.05). The results of our study provide direct evidence that the response of the human detrusor tomechanical stretch is regulated by activation of mechano-gated TREK-1 channels. Impaired mechanosensation and mechanotransduction associated with the changes in stretch-activated K-2P channels may underlie myogenic bladder dysfunction in humans.
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