期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 593, 期 6, 页码 1443-1458出版社
WILEY
DOI: 10.1113/jphysiol.2014.280784
关键词
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资金
- Ohio State University, Department of Physiology & Cell Biology pilot grant
- National Institutes of Health (NIH) [HL074045, HL063043]
- NIH [HL115580]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL074045, R01HL124935, R01HL063043, R01HL115580, R01HL088635] Funding Source: NIH RePORTER
Aberrant diastolic calcium (Ca) release due to leaky ryanodine receptors (RyR2s) has been recently associated with atrial fibrillation (AF) and catecholaminergic polymorphic ventricular tachycardia (CPVT). However, it remains unclear how diastolic Ca release contributes to the rising of rapid repetitive focal activity, which is considered as a common AF triggering mechanism. To address this question, we conducted simultaneous voltage/Ca optical mapping in atrial tissue and one-/two-dimensional confocal imaging in atrial tissue and myocytes from wild-type (WT, n=15) and CPVT mice lacking calsequestrin 2 (Casq2(-/-), n=45), which promotes diastolic Ca release. During -adrenergic stimulation (100nm isoproterenol), only Casq2(-/-)atrial myocytes showed pacing-induced self-sustained repetitive activity (31 +/- 21s vs. none in WT). Importantly, in atrial tissue, this repetitive activity could translate to Ca-dependent focal arrhythmia. Ectopic action potential (AP) firing during repetitive activity occurred only when diastolic Ca release achieved a sufficient level of synchronization. The AP, in turn, synchronized subsequent diastolic Ca release by temporally aligning multiple sources of Ca waves both within individual myocytes and throughout the atrial tissue. This alternating interplay between AP and diastolic Ca release perpetuates the self-sustaining repetitive activity. In fact, pharmacological disruption of synchronized diastolic Ca release (by ryanodine) prevented aberrant APs; and vice versa, the inhibition of AP (by TTX or 0 Na, 0 Ca solution) de-synchronized diastolic Ca release. Taken together, these results suggest that a cyclical interaction between synchronized diastolic Ca release and AP forms a pathological rhythm generator that is involved in Ca-dependent atrial arrhythmias in CPVT.
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