期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 590, 期 16, 页码 3877-3890出版社
WILEY-BLACKWELL
DOI: 10.1113/jphysiol.2012.229013
关键词
-
资金
- National Institutes of Neurological Disorders and Stroke of the NIH [RO1-NS041417, RO1-NS065295]
- Individual National Research Service Award [F32-NS065581]
- [T32-HL007275]
Rapidly activating and rapidly inactivating voltage-gated A-type K+ currents, I-A, are key determinants of neuronal excitability and several studies suggest a critical role for the Kv4.2 pore-forming alpha subunit in the generation of I-A channels in hippocampal and cortical pyramidal neurons. The experiments here demonstrate that Kv4.2, Kv4.3 and Kv1.4 all contribute to the generation of I-A channels in mature cortical pyramidal (CP) neurons and that Kv4.2-, Kv4.3- and Kv1.4-encoded I-A channels play distinct roles in regulating the intrinsic excitability and the firing properties of mature CP neurons. In vivo loss of Kv4.2, for example, alters the input resistances, current thresholds for action potential generation and action potential repolarization of mature CP neurons. Elimination of Kv4.3 also prolongs action potential duration, whereas the input resistances and the current thresholds for action potential generation in Kv4.3(-/-) and WT CP neurons are indistinguishable. In addition, although increased repetitive firing was observed in both Kv4.2(-/-) and Kv4.3(-/-) CP neurons, the increases in Kv4.2(-/-) CP neurons were observed in response to small, but not large, amplitude depolarizing current injections, whereas firing rates were higher in Kv4.3(-/-) CP neurons only with large amplitude current injections. In vivo loss of Kv1.4, in contrast, had minimal effects on the intrinsic excitability and the firing properties of mature CP neurons. Comparison of the effects of pharmacological blockade of Kv4-encoded currents in Kv1.4(-/-) and WT CP neurons, however, revealed that Kv1.4-encoded I-A channels do contribute to controlling resting membrane potentials, the regulation of current thresholds for action potential generation and repetitive firing rates in mature CP neurons.
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