期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 589, 期 22, 页码 5539-5553出版社
WILEY
DOI: 10.1113/jphysiol.2011.215277
关键词
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资金
- NIH [T32AG000268, R21 HL098810, RO1 HL085497]
- Department of Veterans Affairs [CDA-2-0039]
- Baltimore Veterans Affairs Medical Center Geriatric Research, Education, and Clinical Center
- American College of Sports Medicine Foundation
- University of Maryland Department of Kinesiology
We hypothesized that prior exercise would prevent postprandial lipaemia (PPL)induced increases in intracellular reactive oxygen species (ROS) in three distinct circulating angiogenic cell (CAC) subpopulations. CD34(+), CD31(+)/CD14(-)/CD34(-), and CD31+/CD14+/CD34-CACs were isolated from blood samples obtained from 10 healthy men before and 4 h after ingesting a high fat meal with or without similar to 50 min of prior endurance exercise. Significant PPL-induced increases in ROS production in both sets of CD31(+) cells were abolished by prior exercise. Experimental ex vivo inhibition of NADPH oxidase activity and mitochondrial ROS production indicated that mitochondria were the primary source of PPL-induced oxidative stress. The attenuated increases in ROS with prior exercise were associated with increased antioxidant gene expression in CD31(+)/CD14(-)/CD34(-) cells and reduced intracellular lipid uptake in CD31(+)/CD14(+)/CD34(-) cells. These findings were associated with systemic cardiovascular benefits of exercise, as serum triglyceride, oxidized low density lipoprotein-cholesterol, and plasma endothelial microparticle concentrations were lower in the prior exercise trial than the control trial. In conclusion, prior exercise completely prevents PPL-induced increases in ROS in CD31(+)/CD14(-)/CD34(-) and CD31(+)/CD14(+)/CD34(-) cells. The mechanisms underlying the effects of exercise on CAC function appear to vary among specific CAC types.
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