期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 588, 期 2, 页码 315-324出版社
WILEY
DOI: 10.1113/jphysiol.2009.183582
关键词
-
资金
- Belgian Ministry for Science Policy [IUAP P6/28]
- Research Foundation-Flanders [G.0172.03, G.0565.07]
- Research Council of the KU Leuven [GOA 2004/07, EF/95/010]
TRPM8, a cation channel activated by cold and by cooling agents such as menthol and icilin, is critically involved in somatosensory cold sensation. Ion fluxes through TRPM8 are highly sensitive to changes in extracellular Ca2+ and pH, but the mechanisms underlying this type of modulation are poorly understood. Here we provide evidence that inhibition of TRPM8 currents by extracellular divalent cations and protons is due to surface charge screening. We demonstrate that increasing concentrations of divalent cations or protons cause parallel shifts of the voltage dependence of TRPM8 activation towards positive potentials. These shifts were interpreted using the Gouy-Chapman-Stern theory, yielding an estimate for the density of fixed negative surface charge between 0.0098 and 0.0126 equivalent charges per angstrom(2). These results represent the first description of the effects of surface charge screening on a TRP channel and provide a straightforward explanation for the known effects of extracellular Ca2+ on cold-sensitive neurons.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据