期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 588, 期 16, 页码 3031-3043出版社
WILEY
DOI: 10.1113/jphysiol.2009.184705
关键词
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资金
- National Institutes of Health [NS13742]
- Korean Government (MOEHRD) [KRF-2005-213-E00003]
- Ministry of Science and Technology, Republic of Korea
The role of P/Q- and T-type calcium channels in the rhythmic oscillatory behaviour of inferior olive (IO) neurons was investigated in mutant mice. Mice lacking either the Ca(V)2.1 gene of the pore-forming alpha 1A subunit for P/Q-type calcium channel, or the Ca(V)3.1 gene of the pore-forming alpha 1G subunit for T-type calcium channel were used. In vitro intracellular recording from IO neurons reveals that the amplitude and frequency of sinusoidal subthreshold oscillations (SSTOs) were reduced in the Ca(V)2.1-/- mice. In the Ca(V)3.1-/- mice, IO neurons also showed altered patterns of SSTOs and the probability of SSTO generation was significantly lower (15%, 5 of 34 neurons) than that of wild-type (78%, 31 of 40 neurons) or Ca(V)2.1-/- mice (73%, 22 of 30 neurons). In addition, the low-threshold calcium spike and the sustained endogenous oscillation following rebound potentials were absent in IO neurons from Ca(V)3.1-/- mice. Moreover, the phase-reset dynamics of oscillatory properties of single neurons and neuronal clusters in IO were remarkably altered in both Ca(V)2.1-/- and Ca(V)3.1-/- mice. These results suggest that both alpha 1A P/Q- and alpha 1G T-type calcium channels are required for the dynamic control of neuronal oscillations in the IO. These findings were supported by results from a mathematical IO neuronal model that incorporated T and P/Q channel kinetics.
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