期刊
JOURNAL OF PHYSICS-CONDENSED MATTER
卷 26, 期 46, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0953-8984/26/46/463103
关键词
protein structure; multi-protein complexes; hybrid methods of structural biology
资金
- Polish National Science Center [2012/05/B/NZ1/00631]
- MarieCurie FP7-PEOPLE-CIG project [333916]
- Ministry of Education of the Czech Republic [LO1302]
- Academy of Sciences Czech Republic [RVO: 61388963]
Structural biology elucidates atomic structures of macromolecules such as proteins, DNA, RNA, and their complexes to understand the basic mechanisms of their functions. Among proteins that pose the most difficult problems to current efforts are those which have several large domains connected by long, flexible polypeptide segments. Although abundant and critically important in biological cells, such proteins have proven intractable by conventional techniques. This gap has recently led to the advancement of hybrid methods that use state-of-the-art computational tools to combine complementary data from various high-and low-resolution experiments. In this review, we briefly discuss the individual experimental techniques to illustrate their strengths and limitations, and then focus on the use of hybrid methods in structural biology. We describe how representative structures of dynamic multi-protein complexes are obtained utilizing the EROS hybrid method that we have co-developed.
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