期刊
PHYSICAL REVIEW LETTERS
卷 114, 期 13, 页码 -出版社
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevLett.114.138102
关键词
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资金
- Department of Energy, Office of Basic Energy Sciences [DE-SC0010432TDD]
- [NSF-MRI-0923057]
- [NSF-CMMI-1068566]
- [NSF-MCB-1329623]
- [NSF-CMMI-1068852]
- [NSF-MRSEC-1420382]
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1068852] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1329623] Funding Source: National Science Foundation
In the presence of nonadsorbing polymers, colloidal particles experience ubiquitous attractive interactions induced by depletion forces. Here, we measure the depletion interaction between a pair of microtubule filaments using a method that combines single filament imaging with optical trapping. By quantifying the dependence of filament cohesion on both polymer concentration and solution ionic strength, we demonstrate that the minimal model of depletion, based on the Asakura-Oosawa theory, fails to quantitatively describe the experimental data. By measuring the cohesion strength in two-and three-filament bundles, we verify pairwise additivity of depletion interactions for the specific experimental conditions. The described experimental technique can be used to measure pairwise interactions between various biological or synthetic filaments and complements information extracted from bulk osmotic stress experiments.
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