Unzipping of Double-Stranded DNA in Engineered α-Hemolysin Pores

The biological protein a.-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the alpha-hemolysin pore has for a long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the alpha-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing.