High Transfection Efficiency of Homogeneous DNA Nanoparticles Induced by Imidazolium Gemini Surfactant as Nonviral Vector

Nonviral vectors are highly desirable for the development of efficient gene delivery systems. In this study, we report the monomolecular condensation of plasmid DNA and efficient cell transfection by imidazolium gemini surfactants ([C-12-4-C(12)im]Br-2), which could be a potential nonviral vector for efficient gene therapy. Homogeneous DNA/[C-12-4-C(12)im]Br-2 nanoparticles are formed with a diameter of approximately 100 nm and investigated by using atomic force microscopy. DNA condensates evolve from supercoiled DNA molecules, to individual toroids, to close-packed particles, and eventually to multimolecular aggregates with the increase of [C-12-4-C(12)im]Br-2 concentrations. Highly efficient gene transfection in vitro is demonstrated in human embryonic kidney 293 (HEK293) and HeLa cells, which could be attributed to the effective DNA condensation into uniform nanoparticles induced by [C-12-4-C(12)im]Br-2. In addition, the low cytotoxicity of [C-12-4-C(12)im]Br-2 at transfection concentration region verified by cell viability assay (3[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, MTT assay) also supports [C-12-4-C(12)im]Br-2 as an effective gene vector. The high gene transfection efficiency by [C-12-4C(12)im]Br-2 as well as its low cytotoxicity could shed light on the rational molecular design of nonviral vectors for gene delivery systems.