期刊
JOURNAL OF PHYSICAL CHEMISTRY C
卷 117, 期 30, 页码 15677-15684出版社
AMER CHEMICAL SOC
DOI: 10.1021/jp403946x
关键词
-
资金
- University of Waterloo
- Canadian Foundation for Innovation
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Canadian Institutes of Health Research (CIHR)
- Early Researcher Award from the Ontario Ministry of Research and Innovation
- Netherlands Technology Foundation STW (STW-Project) [11818]
We have recently reported on the fast and quantitative adsorption of DNA to 13 nm gold nanoparticles (AuNPs) at pH 3. This is in contrast to most traditional methods at neutral pH, where the adsorption is both slow and requires high excess of DNA. Direct application of our protocol to large particles in many cases did not result in particles that are stable at high (0.3 M) salt, and high excess of DNA was still required for the formation of stable particles. In this work, we investigate the reasons for this limitation on the basis of kinetics and colloidal stability. On the basis of our investigation, fast and quantitative modification of large AuNPs is still possible, either by working at high particle concentration, or by using sonication. As we have shown that fast quantitative modification of large particles is possible, the preparation step of reduction and purification of the thiolated DNA becomes the rate limiting step in the whole AuNP-DNA conjugate protocol. However, we show that this step is unnecessary when using our current protocol.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据